SECTION
1
PLANT
SCENTS
|
A
|
Everyone is
familiar with scented flowers, and many people have heard that floral odors
help the plant attract pollinators. This common notion is mostly correct,
but it is surprising how little scientific proof of it exists. Of course,
not all flowers are pollinated by biological agents— for example, many
grasses are wind- pollinated—but the flowers of the grasses may still emit
volatiles. In fact, plants emit organic molecules all the time, although
they may not be obvious to the human nose. As for flower scents that we can
detect with our noses, bouquets that attract moths and butterflies generally
smell “sweet,” and those that attract certain flies seem “rotten” to us.
|
B
|
The release
of volatiles from vegetative parts of the plant is familiar, although until
recently the physiological functions of these chemicals were less clear and
had received much less attention from scientists. When the trunk of a pine
tree is injured- for example, when a beetle tries to burrow into it- it
exudes a very smelly resin. This resin consists mostly of
terpenes—hydrocarbons with a backbone of 10,15 or 20
carbons that may also contain atoms of oxygen. The heavier C20 terpenes, called
diterpenes, are glue-like and can cover and immobilize insects as they plug
the hole. This defense mechanism is as ancient as it is effective: Many
samples of fossilized resin, or amber, contain the remains of insects
trapped inside. Many other plants emit volatiles when injured, and in some
cases the emitted signal helps defend the plant. For example,(Z)_3_ hexenyl
acetate, which is known as a “green leaf volatile” because it is emitted by
many plants upon injury, deters females of the moth Heliothis virescens
from laying eggs on injured tobacco plants. Interestingly, the profile of
emitted tobacco volatiles is different at night than during the day, and it
is the nocturnal blend, rich in several (Z) 3_hexen_i-olesters, that is
most effective in repelling the night-active H. virescens moths.
|
C
|
Herbivore
induced volatiles often serve as indirect defenses. These bulwarks exist in
a variety of plant species, including corn, beans, and the model plant
species Arabidopsis thaliana. Plants not only emit volatiles acutely, at
the site where caterpillars, mites, aphids or similar insects are eating
them, but also generally from non-damaged parts of the plant. These signals
attract a variety of predatory insects that prey on the plant-eaters. For example,
some parasitic wasps can detect the volatile signature of a damaged plant
and will lay their eggs inside the offending caterpillar; eventually the
wasp eggs hatch, and the emerging larvae feed on the caterpillar from the
inside out. The growth of infected caterpillars is retarded considerably,
to the benefit of the plant. Similarly, volatiles released by plants in
response to herbivore egg laying can attract parasites of the eggs, thereby
preventing them from hatching and avoiding the onslaught of hungry herbivores
that would have emerged. Plant volatiles can also be used as a kind of
currency in some very indirect defensive schemes. In the rainforest understory
tree Leonardoxa africana, ants of the species Petalomyrmex phylax patrol
young leaves and attack any herbivorous insects that they encounter. The young
leaves emit high levels of the volatile compound methyl salicylate, a
compound that the ants use either as a pheromone or as an antiseptic in
their nests. It appears that methyl salicylate is both an attractant and a
reward offered by the tree to get the ants to perform this valuable
deterrent role.
|
D
|
Floral scent
has a strong impact on the economic success of many agricultural crops that
rely on insect pollinators, including fruit trees such as the
bee-pollinated cherry, apple, apricot and peach, as well as vegetables and tropical
plants such as papaya. Pollination not only affects crop yield, but also
the quality and efficiency of crop production. Many crops require most, if
not all, ovules to be fertilized for optimum fruit size and shape. A
decrease in fragrance emission reduces the ability of flowers to attract pollinators
and results in considerable losses for growers, particularly for introduced
species that had a specialized pollinator in their place of origin. This
problem has been exacerbated by recent disease epidemics that have killed
many honeybees, the major insect pollinators in the United States.
|
E
|
One means by
which plant breeders circumvent the pollination problem is by breeding self-compatible,
or apomictic, varieties that do not require fertilization. Although this solution
is adequate, its drawbacks include near genetic uniformity and consequent susceptibility
to pathogens. Some growers have attempted to enhance honeybee foraging by
spraying scent compounds on orchard trees, but this approach was costly,
had to be repeated, had potentially toxic effects on the soil or local biota,
and, in the end, proved to be inefficient. The poor effectiveness of this
strategy probably reflects inherent limitations of the artificial,
topically applied compounds, which clearly fail to convey the appropriate message
to the bees. For example, general spraying of the volatile mixture cannot
tell the insects where exactly the blossoms are. Clearly, a more refined
strategy is needed. The ability to enhance existing floral scent, create
scent de novo or change the characteristics of the scent, which could all
be accomplished by genetic engineering, would allow us to manipulate the
types of insect pollinators and the frequency of their visits. Moreover,
the metabolic engineering of fragrance could increase crop protection
against pathogens and pests.
|
F
|
Genetic
manipulation of scent will also benefit the floriculture industry.
Ornamentals, including cut flowers, foliage and potted plants, play an important
aesthetic role in human life. Unfortunately, traditional breeding has often
produced cultivars with improved vase life, shipping characteristics, color
and shape while sacrificing desirable perfumes. The loss of scent among
ornamentals, which have a worldwide value of more than $30 billion, makes
them important targets for the genetic manipulation of flower fragrance. Some
work has already begun in this area, as several groups have created petunia
and carnation plants that express the linalool synthase gene from C.
Breweri. These experiments are still preliminary: For technical reasons,
the gene was expressed everywhere in the plant, and although the transgenic
plants did create small amounts of linalool, the level was below the
threshold of detection for the human nose. Similar experiments in tobacco
used genes for other monoterpene synthases, such as the one that produces
limonene, but gave similar results.
|
G
|
The next
generation of experiments, already in progress, includes sophisticated schemes
that target the expression of scent genes specifically to flowers or other organs—such
as special glands that can store antimicrobial or herbivore- repellent compounds.
|
Questions 1-4
The reading Passage has seven
paragraphs A-G.
Which paragraph contains the following
information?
Write the correct letter A-G, in boxes
1-4 on your answer sheet.
1
|
Substance released to help plants themselves.
|
2
|
Scent helps plant’s pollination.
|
3
|
Practice
on genetic experiment of fragrance.
|
4
|
Plant’s scent attracts herbivore’s enemy for protection.
|
Questions 5-8
Do the following statements agree with
the information given in Reading Passage 1? In
boxes 5-8 on your answer sheet, write
TRUE
|
if the statement is true
|
FALSE
|
if the
statement is false
|
NOT GIVEN
|
if the
information is not given in the passage
|
5
|
We have few evidence to support the idea that scent attracts
pollinators.
|
6
|
Heliothis virescens won’t eat those tobacco leaves on which they
laid eggs.
|
7
|
Certain
ants are attracted by volatiles to guard plants in rainforest.
|
8
|
Pollination only affects fruit trees' production rather than other
crop trees.
|
Questions 9-13
Choose the correct letter, A, B,
C or D.
Write your answers in boxes 9-13 on
your answer sheet.
9
|
How do wasps
protect plants when they are attracted by scents according to the passage?
|
A
|
plants induce
wasps to prey herbivore.
|
B
|
wasps lay eggs into
caterpillars.
|
C
|
wasps laid
eggs on plants to expel herbivore.
|
D
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offending caterpillars and wasp
eggs coexist well.
|
10
|
What reason
caused number of honeybees decline m the United States.
|
A
|
pollination process
|
B
|
spread
illness
|
C
|
crop trees are poisonous
|
D
|
grower's
overlook
|
11
|
Which of the following
drawbacks about artificial fragrance is NOT mentioned in the passage?
|
A
|
it’s very
expensive
|
B
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it can't tell correct
information to pollinators.
|
C
|
it needs
massive manual labour
|
D
|
it poisons local environment
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12
|
The number of
$30 billion quoted in the passage is to illustrate the fact that:
|
A
|
favorable perfumes are made
from ornamental flowers
|
B
|
traditional
floriculture industry needs reform.
|
C
|
genetic operation on scent can
make vast profit.
|
D
|
Scent plays a
significant role in Ornamental industry.
|
13
|
What is weakness of genetic
experiments on fragrance?
|
A
|
Linalool
level is too low to be smelt by nose
|
B
|
no progress made in linalool
emission
|
C
|
experiment on
tobacco has a better result
|
D
|
transgenic plants produce
intense scent
|
|
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